Methods ASYD-2::GFP reporter was constructed and used as an active zone marker for forward genetic screen to identify genetic lociaffecting the active zone formation.
方法我们通过构建SYD-2::GFP转基因动物作为活动区的标记进行正向遗传筛选,以鉴定影响活动区形成的系列遗传位点。
Becallse of the difficulties in technology,the application of genetic screen in mammalians is not widespread.
遗传筛选在模式生物系统(如酵母、线虫、果蝇和拟南芥)中一直是一种非常有效的研究手段。