The produced multi-copy was then cloned into pUC18 vector.
目的: 构建抗菌肽Aurein1 2基因多拷贝串联体,并将目的基因串联产物克隆到载体pUC18上。
In this paper, the multi-copy expression vectors containing Penicillum expansum lipase(PEL) gene-lip were constructed in vitro and then the multi-copy engineered strains were obtained after the vectors were transformed into Pichia pastori by electroporation, the higher-level expression of lip was achieved in Pichia pastoris.
本研究采用细胞外基因操作方法构建了扩展青霉碱性脂肪酶(Penicillium expansum lipase，PEL)多拷贝表达质粒，并将表达质粒转化巴斯德毕赤酵母构建成多拷贝基因工程菌，有效地提高了PEL在巴斯德毕赤酵母中的表达水平。
Construction of acidic peptide-fused multi-copy antimicrobial peptide tandem in the same direction;
为提高抗菌肽的表达 ,设计在抗菌肽基因的N端融合一段编码酸性肽的片段以减轻表达产物对宿主的毒性 ,通过含有酶切位点的接头将该融合肽基因以同向串连的方式连接成多拷贝基因 ,克隆至pUC19载体。
Analysis of Differential Expression of Rol Gene Multi-copied in the Poplar
多拷贝rol基因在杨树中表达差异分析
Design, Expression and Purification of Tandem Multimers of Peptide Antibiotic hPAB-β;
肽抗生素hPAB-β多拷贝串联体的设计、表达与纯化
Multimerization and Fusion Expression of Antimicrobial Peptide LfcinB15-W4, 10;
抗菌肽LfcinB15-W4，10多拷贝表达载体的构建及融合表达
Genetic polymorphism of the multi-copy Y-STR loci DYS385,DYS459 and DYS464 in Wuhan Han population;
武汉汉族群体3个多拷贝Y-STR基因座的遗传多态性
The Construction of Engineered Strains Containing Multiple Lip Copies and Site-directed Mutagenesis of Psychrophilic Lipase;
多拷贝基因工程菌的构建及低温脂肪酶的定点突变
Expression of Osteopontin RGD Peptide in E.coli and Its Biological Activities;
骨桥蛋白RGD多拷贝短肽的表达、纯化及生物学活性研究
Construction of Expression Vectors in T.reesei with Multicopies Strategy;
多拷贝策略构建瑞氏木霉外源基因系列表达载体
Construction of a Multi-copy Integration Vector for Saccharomyces-cerevisiae Expressing Salmon Calcitonin and Safety Evaluation;
鲑鱼降钙素在酵母中的多拷贝表达及安全性评价
The Optimization of CCR PCR Conditions,the New Discovery and Analysis of Multi-copy Gene in the Genus Corymbia;
伞房属CCR基因PCR优化体系建立及多拷贝的发现和分析
Construction and Eukaryotic Expression of a Series of Fusion Genetic Fragments Encoding Multi-Copies Heterological Prostate Stem Cell Antigen
多拷贝异种化前列腺干细胞抗原融合基因片段的构建及真核表达
multiple antigenic peptide
多抗原肽[以赖氨酰残基作内核而以多拷贝的肽抗原作外表层的分枝状合成肽]
This eliminates the multiple interrupts of packet copying.
这就消除了包拷贝的多个中断。
FcgRIIIb Gene Copy Number Polymorphism Predisposes to Susceptibility of IgAN in Chinese;
IgA肾病与Fcgr3B基因拷贝数多态性研究
Copy-number variation:a new pattern of structural diversity in genome
拷贝数变异:基因组多样性的新形式
Since the beginning of the 1990s a total of more than 630 films in upwards of 8,500 copies have been dubbed in Tibetan.
九十年代以来累计译制藏语影片630多部，8500多个拷贝。
It replaces the hard-copy approach with an electronic format that permits a much higher level of interaction.
这是以一种允许高得多的交互级的电子格式来替代硬拷贝方式。
Batch-size certificate released by Microsoft is an easy way to purchase software copies in large quantities.
微软的批量许可证是购买多个软件拷贝的简易方法。
However, most people who have seen the movie say these film copies are a much better quality than other filmed movies.
然而，多数观众观后认为即使这些拷贝仍比用胶片拍的电影质量高。
