The internal ribosome entry site (IRES) from encephalomyocarditis and that from polio viruses were used to construct a polycistronic retroviral vector pGCEN/TRI, where Neoresistant gene was used as a marker gene and TNFα cDNA, IL2 cDNA as genes of interest, so that the LTR promoter derived the expression of a tricistronic mRNA.
利用脑炎心肌炎病毒和脊髓灰质炎病毒的内核糖体进入位点，连接人ＴＮＦ－α及ＩＬ－２ｃＤＮＡ和选择基因新霉素磷酸转移酶基因（ＮｅｏＲ），使ＴＮＦ－α、ＩＬ－２及ＮｅｏＲ基因均受控于病毒ＬＴＲ启动子，将这３个基因转录至同一ｍＲＮＡ，从而构建成含人ＴＮＦ－α、ＩＬ－２及ＮｅｏＲ基因多顺反子逆转录病毒载体ｐＧＣＥＮ／ＴＲＩ。
Construction and Identification of Polycistron Adenoviral Expression Vector PCA_(13)/FasL-IRES-iNOS;
多顺反子腺病毒表达载体PCA_(13)/FasL-IRES-iNOS的构建和鉴定
The genes were assembled to give polycistronic structures, and under the control of a strong araBAD promoter, the recombinant strain TaraCRH was obtained.
通过PCR分别扩增得到N-carbamoylase基因和上游带有RBS区的D-hydantoinase基因,并将其依次克隆入pBAD/HisA质粒中,得到呈多顺反子结构的双酶表达质粒pBAD-CRH,转化E。
A recombinant plasmid pSE-gpd1-hor2 containing polycistron was constructed under the control of the strong trc promoter.
从酿酒酵母(Saccharomycescerevisiae)克隆3_磷酸甘油脱氢酶基因(gpd1)和3_磷酸甘油酯酶基因(hor2 ) ,并将两个基因串连到启动子trc的下游,构建由trc启动子控制的能高效表达的多顺反子重组质粒pSE_gpd1_hor2 ,将重组质粒导入大肠杆菌BL2 1菌株中,构建得到的重组菌株GxB_gh能将葡萄糖转化为甘油。
Construction of Chloroplast Multicistron Single-cross Expression Vector in Brassica napus L.;
甘蓝型油菜叶绿体多顺反子单交换表达载体构建
Construction of Rice Chloroplast Multicistron Site Integration Expression Vector and Its Transgene to Tobacco Chloroplast;
水稻质体多顺反子表达载体的构建及其对烟草质体的转化
Construction of Tomato Chloroplast Multicistron Site Integration Expression Vector and Its Transgenic Tobacco;
番茄质体多顺反子定点整合表达载体的构建及其转烟草的研究
New Strategy for Construction of Polycistron Expression Vector
构建多顺反子表达载体系统的新策略
intercistronic regio
顺反子间区[多顺反子转录单位中一个基因的终止密码子与下一个基因的起始密码子之间区域]
Construction of Multicistron Expression Vector by Using Plastid Homologous Sequences of Sweet Potato
利用甘薯质体同源片段构建多顺反子表达载体
Construction of Eukaryote Polycistronic Expression Vector and Expression in Hela;
真核生物多顺反子表达载体的构建及在Hela细胞中的表达
Construction of The Safely and Friendly Environmental Tobacco Chloroplast Multicistron Site Integration Expression Vector;
安全环保的烟草质体多顺反子定点整合表达载体的构建
Multicistronic Lentiviral Vector Mediated High Expression of Encoded Genes in Human Embryo Stem Cell
多顺反子慢病毒载体介导编码基因在人胚胎干细胞中的表达
monocistronic messenger RNA
单顺反子信使ＲＮＡ
polycistronic messenger RNA
顺反子信使 rna
THE ELECTRONIC STRUCTURES AND BIOLOGICAL? ACTIVITIES OF METHOMYL′S CIS? ?AND TRANS?ISOMERS
灭多威顺、反异构体的电子结构和生物活性
one cistron-one subunit concept
一顺反子一亚基概念
Application of the cis-trans test defines the genetic unit of function, the cistron.
顺?反测验可以用来确定遗传的功能单位――顺反子。
CIS /TRANS ISOMERIC BONDING PARAMETER TOPOLOGICAL INDEX AND THEIR APPLICATION IN CORRELTION WITH THE PROPERTIES OF CIS /TRANS ISOMERS
顺反异构键参数拓扑指数及其在相关顺反异构分子性质中的应用
reflection type electron paramagnetic resonance spectrometer
反射式电子顺磁共振波谱仪
The cis-trans isomerization reaction has been shown to be a first order reaction with an activation energy of 19 kcal/mole.
顺-反异构化反应系属一极反应,反应活化能为19千卡/克分子。
magnetic ratchet multi-purpose screw-driver with plastic handle
磁性塑料柄倒顺多用螺丝起子
After being touched up, the essay reads more smoothly.
稿子经过这样一改， 念起来就顺口多了。
It's easier to swim with the tide than to oppose the views of the majority.
顺应潮流要比反对大多数人的意见来得容易。
All that she did do was to attend to his wants quietly, and with a humility which was in striking contrast to her former imperious bearing.
她只是一反往常盛气凌人的样子，恭顺地侍候他。
