Developing of The BVgD-Peptide ELISA for Detection of Antibodies Against Monkey B Virus;
猴B病毒BVgD-多肽ELISA检测方法的建立
A Survey on Infection Rate of BV in Rhesus Monkeys by Serologic Antibody;
恒河猴B病毒血清抗体流行病学调查
Study on the Regulation of BV Antibody Level of Captive-bred Macaca fascicularis;
人工驯养条件下食蟹猴B病毒抗体水平变化规律研究
Identification of Herpesvirus simiae (B virus) by TD-PCR and Restriction Enzyme;
应用TD-PCR技术和限制性内切酶技术鉴定猴B病毒
B virus is one of P4 zoonotic agent, which can cause fatal encephalomyelitis in humans.
B病毒是一种P4级传染性病原,能引起人脑脊髓炎,其天然宿主为猕猴,B病毒侵染猕猴后无症状或症状轻微,但它却能致人死亡。
Objective Enzyme immunoassay (EIA) for detection of B virus antibodies in rhesus monkey sera was developed using B virus (BV) antigen.
目的 建立B病毒玻片免疫酶法 (BVEIA)。
Study on synergistic action of Bacillus thuringiensis to Mamestra brassicae nuclear polyhedrosis virus;
苏云金杆菌对甘蓝夜蛾核型多角体病毒增效作用的研究
A pair of primers were designed and synthesized based on the nucleotide sequence of the coat protein gene of chrysanthemum virus B (CVB).
根据菊花B病毒 (CVB)的外壳蛋白基因序列设计、合成引物 ,以感病组织和健康组织总RNA为模板 ,进行cDNA合成和PCR扩增 ,结果从感病组织中扩增出与预期的 776bp大小一致的目标片段 ,而健康组织无此扩增产物 ;将PCR产物插入pGEM -T载体克隆并测序 ,序列分析表明与CVB的相应序列同源性达到 85 % ;将感病组织总RNA以 10x梯度稀释成不同浓度 ,测出RT -PCR检测CVB的灵敏度为 10 -4x ;PCR产物克隆作为RT -PCR反应的阳性对照 ,解决了毒源保存和传播的问题 ,从而建立了CVB快速、灵敏、准确的RT -PCR鉴定和检测技
Grapevine Corky Bark (GCB) is a disease that caused by Grapevine Virus B (GVB), which made a great harm to grapevine species and cultivars.
本研究的目的是建立快速、准确、实用的葡萄病毒B的检测试剂盒,以期解决种苗检疫、抗病性早期鉴定等基本问题,为葡萄无毒化栽培提供基本保障,因而葡萄病毒B的检测技术研究具有非常重要的现实意义。
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