Feasibility of donor cells marked by green fluorescent protein;
绿色荧光蛋白作为供体细胞标记物的可行性
Effects of derivation and pretreatment of donor cells on the efficiency of nuclear transfer in cattle;
供体细胞来源及预处理对牛体细胞核移植效率的影响
Dissociation and culture research of porcine donor cell and cell subculture in somatic cell nuclear transfer;
猪核移植供体细胞的分离培养及传代的研究
To establish human lactoferrin cDNA transgenic donor cell lines and prepare competent donor cells for the production of transgenic animals by somatic cell nuclear transfer (SCNT) and detect the tissue-specific expression of human lactoferrin cDNA, Human lactoferrin cDNA was obtained by RT-PCR while 6.
脂质体介导法转染山羊胎儿成纤维细胞,G418抗性筛选3-4周后,经PCR扩增和报告基因EGFP表达检测,得到稳定整合外源基因的转基因供体细胞系;同时在稳定转染p6。
To establish humanβ-defensin-3 gene transgenic cell lines as competent donor cells for the production of transgenic animals using somatic cell nuclear transfer (SCNT).
最终将构建成功的表达载体通过脂质体法转染奶牛胎儿成纤维细胞和乳腺上皮细胞,转染24-48 h后,在倒置显微镜下观察转染效率;用G418进行抗性细胞克隆的药物筛选,2周后,提取G418抗性细胞克隆的基因组DNA和细胞总RNA,用PCR和RT-PCR方法检测出阳性转基因细胞克隆,鉴定转基因细胞是否整合到细胞的基因组中,以便最终为通过乳腺生物反应器生产药用蛋白的转基因动物提供核移植供体细胞,然后扩大培养,冻存阳性细胞,为下一步进行核移植提供供体细胞;同时,将构建的表达载体转染奶牛乳腺上皮细胞,筛选阳性克隆进行扩大培养,加入胰岛素,氢化可的松和催乳素进行诱导表达。
One of the most prospect field is bacterial vector vaccine.
细菌载体疫苗是新型疫苗的重要发展方向,文章主要对卡介苗、志贺菌和乳酸杆菌等细菌载体疫苗加以介绍。
Donor cells play a very important role in nuclear transplantation of Somatic Cell.
供体细胞在体细胞核移植中发挥着非常重要的作用。
Development of Embryos Reconstructed with Induced Enucleation Oocytes and Mouse Fetal Fibroblast Cells;
小鼠化学去核卵母细胞支持供体细胞核体外发育的研究
Establishment of Donor Somatic Cell Lines and Oocyte in Vitro Maturation and Parthenogenetic Activation of Putian Black Pig
莆田黑猪供体细胞系建立及卵母细胞体外成熟和孤雌激活
The Influence of HMG、EGF、TGF-α on IVM and Donor Cell on Nuclear Transfer Embryos of Bovine;
HMG、EGF、TGF-α对牛卵母细胞IVM及供体细胞对重构胚的影响
Preparation of Donor Nuclei by Immunosurgery and Application in Bovine Somatic Cell Nuclear Transfer
免疫外科法制备牛供体细胞核胞体及其核移植研究
The Selection of Nuclear Donor Cell in Therapeutic Cloning;
治疗性克隆核供体细胞选择的实验研究
Effects of Allogeneic Donor Apoptotic Cell Transfusion on Recipient Peripheral Blood on Cytokines
输入供体凋亡细胞对受体外周血细胞因子的影响
Intrathymic injection of donor-strained splenocytes on the limb transplantation survival time
供体脾细胞胸腺注射在大鼠肢体移植中的应用
Effects of allogeneic donor apoptotic cell infusion on recipient peripheral immune tolerance
输入供体凋亡细胞对受体外周免疫耐受的影响
Detection and Analysis of Donor Stem Cells after Limbal Stem Cell Transplantation in New-Zealand Rabbits;
新西兰白兔角膜缘干细胞移植后供体干细胞的检测与分析
The precursor or stem cell of a plasma cell.
成浆细胞浆细胞的前体细胞或干细胞
The haploid pollen cells develop in the tapetal zone from spore mother cells.
单倍体花粉细胞由绒毡层提供营养发育。
Preliminary Study of Maternal HLA-haploidentical Hematopoietic Stem Cell Transplantation;
母供体半相合造血干细胞移植的初步研究
ALT711 modulates the functional activities of bone marrow derived endothelial progenitor cells in different aged rats
ALT711干预对高龄大鼠供体内皮祖细胞的活化作用
Under appropriate conditions, the adherent cells from HUCB could form the feeder layer to support long-term hematopoiesis and provide the supporting stromal cells for the ex vivo expansion of HSPCs.
在适当的条件下,可以形成饲养层细胞,为造血干/祖细胞体外扩增提供支持基质细胞.
Induction of Rat Limb Allotransplantation Tolerance by Donor Specific Spleen Cell Tranfusion;
供体脾细胞输注诱导大鼠异体肢体移植耐受的实验研究
Effects of Killer Immunoglobulin-like Receptor and Human Leukocyte Antigen ClassⅠ Ligand on the Prognosis of Related Donor Hematopoitic Stem Cell Transplantation
杀伤细胞免疫球蛋白样受体及其配体对亲缘供者造血干细胞移植预后的影响
Study on Establishing Transgenic Animals by Germ Cells as Exogenous Gene Vector for Xenotransplantation;
以生殖细胞为外源基因载体建立异种移植转基因供体动物的研究
