Effect of p53 gene knockout on cell migration;
p53基因敲除对细胞迁移的影响
Analysis of focal cerebral blood flow,ATP content and protein sythesis of L1 gene knockout mice;
L1CAM基因敲除小鼠的脑局部血流量、ATP含量和蛋白合成率分析
Establishment of a βB2 crystallin gene knockout mice model;
βB2晶体蛋白基因敲除小鼠模型的建立
Breeding,reproducing and identifying for aquaporin-3 gene knock-out mice;
水通道蛋白3基因敲除小鼠的饲养繁殖及鉴定
Gene knock-out technology and its application in the research of c-Jun NH2-Terminal Kinase-2
基因敲除技术及其在JNK-2研究中的应用
Construction and characterization of a two-component signal transduction system ciaR gene knock-out mutant of Streptococcus suis serotype 2
猪链球菌2型二元信号转导系统ciaR基因敲除突变株的构建及生物学功能研究
Immortalization of embryonic fibroblasts in heat shock transcription factor 1 knockout mouse;
HSF1基因敲除小鼠胚胎成纤维细胞的永生化
Effect of Smad4 Knockout on Hearing and Vestibular Function of Mouse;
Smad4基因敲除对小鼠听力和前庭功能的影响
Experimental observations on the physiology of hearing and cochlea morphology in Smad5 knockout mice;
Smad5基因敲除小鼠听生理和耳蜗形态实验观察
Effects of protein tyrosine phosphatase α knock out on Hippocampal CA1 synaptic plasticity;
PTPα基因敲除对小鼠海马CA1区突触可塑性的影响
Objective To study the role of Schwann cells apoptosis in the pathogenesis of experimental autoimmune neuritis(EAN) in TNF receptor Ⅰ knock out(TNFR Ⅰ-/-)mice.
目的:建立实验性自身免疫性神经炎(EAN)在肿瘤坏死因子α受体I(TNFRⅠ)基因敲除鼠(TNFRⅠ-/-)的动物模型,进一步研究雪旺氏细胞凋亡在其发病机制中的作用。
7kb exon6 to exon7 DNA sequence which is separated by PCR as Short Arm(SA),were used to construct HPRT gene knock out vector.
根据已知大鼠HPRT基因的外显子序列,从大鼠HPRT基因BAC中用酶切和PCR方法分别分离得到用于构建基因敲除载体的3。
The gene disruption cassette combined the het- erologous dominant kanr resistance marker with a Cre/loxP-mediated marker removal procedure.
重组质粒pSH-CUP的构建,不仅解决了酵母转化子筛选标记问题和非酵母基因的引入,而且使LoxP-kanMX-loxP基因敲除体系在进行真核生物基因敲除时更加方便可行。
In this thesis,applying the Cre/LoxP system gene disruption method we disrupt the glutamate dehydrogenaseⅠgene(GDH1) from a diploid industry yeast strain SS04 and construct a strain named SS15 of mutating an allele of GDH1, only leaving a single loxP site at the chromosomal locus of GDH1 ORF.
本文以酿酒酵母SS04出发,采用Cre/LoxP系统基因敲除法对酵母谷氨酸脱氢酶Ⅰ基因(GDH1)进行基因敲除操作,构建了一个GDH1等位基因缺失的酿酒酵母菌株SS15,并在原GDH1位置留下了一个LoxP位点。
Constract of Myostatin Knockout Porcine Fetal Fibroblast and Preliminary Study on Myostatin Knockout of Porcine;
MSTN基因敲除猪胚胎成纤维细胞的构建及其基因敲除猪的初步研究
Renal Phenotype of Aging UT-B Knockout Mice and Adult UT-A2/UT-B Knockout Mice
老年UT-B基因敲除和成年UT-A2/UT-B基因敲除鼠肾脏表型研究
Pregnant Phenotype Analysis of AQP3 Null Mice and AQP8 Null Mice
AQP3基因敲除鼠及AQP8基因敲除鼠妊娠相关表型的研究
Application of Cre-LoxP System in The Gene Knockout of Bacillus anthracis and The Knockout of eag Gene
Cre-LoxP系统在炭疽芽胞杆菌基因敲除中的应用及eag基因的敲除
Human Carcinoma of Esophageal Eca9706 Cell DNA Polymerseβ Gene Targeting;
食管癌细胞Eca9706 DNA聚合酶β基因敲除
Functional Characterization of Fusarium Graminearum PTC1 Gene;
禾谷镰刀菌PTC1基因敲除及功能初探
The Impacts of Kir6.2 Knockout on MPTP-induced Acute Neurotoxicity;
Kir6.2基因敲除对MPTP急性神经毒性的影响
Mechanisms of Cleft Palate Formation in Wnt5a Gene Knockout Mouse;
Wnt5a基因敲除小鼠腭裂发生的机制研究
Functional Characterization of Fusarium Graminearum TEP1 Gene
禾谷镰刀菌TEP1基因敲除及功能研究
Study on Development and Mechanism of Arterial Calcification in Adiponectin-deficient Mice
脂联素基因敲除小鼠血管钙化的研究
Construction of Knockout Vector for sIgM λ Light Chain Gene from DT40 Cells
DT40细胞sIgMλ轻链基因敲除载体的构建
The effect of lipoprotein lipase gene on lipid metabolism
脂蛋白脂酶基因敲除鼠的脂代谢研究
Deletion of Bmi-1 gene leads to skin aging in mice
Bmi-1基因敲除导致小鼠皮肤老化
Construction of the Knockout Lines of Heart Development Candidate Gene CG3295 in Drosophila
建立果蝇心脏发育候选基因CG3295的基因敲除系
GENOTYPE ANALYSIS OF FMR1 GENE KNOCKOUT MICE WITH POLYMERASE CHAIN REACTION
采用PCR方法检测Fmr1基因敲除小鼠的基因型
Breeding,Reproducing,and Identifying for PAX-8 Gene Knock-out Mice
PAX-8基因敲除小鼠的饲养繁殖及基因型鉴定
Construction of the Mouse Enamel Matrix Serine Proteinase Gene Targeting Vector;
小鼠釉基质丝氨酸蛋白酶基因敲除载体的构建
Study on Biological Properties of 1α Hydroxyrase Gene Knock-out Mice and Vitamin D3 Receptor Gene Knock-out Mice;
1α羟化酶基因敲除小鼠和维生素D3受体基因敲除小鼠生物学特性的研究
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