Research on the plaque forming features and pathogenicity of H5N1 avian influenza virus strains isolated from human;
人源H5N1亚型禽流感病毒株空斑特性和致病性研究
A new method counting virus plaque form unit (PFU) called plaque counting technic by labeled antibodies staining (PCTLAS) was established.
用鸡马立克氏病病毒血清1、2、3型毒株及其对应的异硫氢酸荧光素(FITC)标记的型特异单克隆抗体(单抗)和型共同单抗为试验材料,以免疫荧光抗体技术(FA)为基础,并加以改进,建立了标记抗体染色病毒空斑计数技术。
Then,eluted the f2 phages from immunomagnetic beads for Plaque assay and observed the elution effects at different pH after being absorbed.
方法:将兔抗f2噬菌体IgG与微磁珠化学偶联构建免疫磁珠,吸附水中f2噬菌体,然后洗脱免疫磁珠结合的噬菌体,空斑实验检测f2噬菌体的效价。
New spatio-temporal patterns including oscillating square pattern,modulated stripe pattern and spiral pattern have been found in a three-variable Brusselator model.
利用扩展的三变量布鲁塞尔模型,得到了新的时空斑图,其中包括振荡四边形斑图、调制条纹斑图以及螺旋波斑图。
BmNPV GD isolate from China was plaque-purified and four bro genes were cloned termed as bro-a,b,c,d.
对BmNPV广东株进行了空斑纯化,并对该基因组的bro基因家族进行克隆,获得4个bro基因序列(bro-a、bc、、d),与GenBank数据库中BmNPVbro基因序列及本实验室测定的重庆株的bro基因序列进行比较分析,结果表明广东株BmNPVbro基因存在插入及缺失,其氨基酸的改变主要发生在对应蛋白的N端部分;同时进行的bro基因的系统发生分析表明,广东株bro基因分别位于3个亚组中,广东株bro-d与日本T3、重庆CQ1株bro-d以及法国SC7株bro-Ⅲ属于亚组A,广东株bro-a、c与T3、CQ1株bro-a、c以及SC7株bro-Ⅱ属于亚组B,广东株bro-b与T3、CQ1株bro-b、e以及SC7株bro-Ⅰ属于亚组C,bro基因进化与地理位置的相关性不明显。
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