Secretive expression of the fusion protein HSA-CNP in Pichia pastoris;
人C-型利钠肽-血清白蛋白融合蛋白质的构建及在毕赤酵母中的分泌表达
PurposeTo prepare m 4AChR-Gilα fusion protein in Baculovirus-Sf 9 cells system and to detect the effects of various muscarinic ligands on the interaction between m 4AChR and Gilα in the to fusion protein, then to screen ligands specific for m 4AChR.
目的通过Baculovirus Sf9细胞系统制备并表达m4AChR Gilα融合蛋白 ,检测几种配体对融合蛋白质中m4AChR与Gilα间相互作用 ,并以该融合蛋白质为工具 ,筛选m4AChR的特异性配体。
To express the fusion protein ATF PAI2CD (urokinase type plasminogen activator amino terminal fragment plasminogen activator inhibitor type 2 with the region inter C and D helices deleted ) gene in E.
为了解尿激酶型纤溶酶激活物 (urokinase typeplaminogenactivator,uPA)的氨基末端片段 (amino terminalfrag ment,ATF)和纤溶酶激活物抑制剂 2 (plasminogenactivatorinhibitortype 2 ,PAI 2 )突变体PAI 2CD融合蛋白在大肠杆菌的表达情况及进一步研究其生物学活性、将ATF PAI2CD融合基因与大肠杆菌表达载体pLY 4重组得到表达质粒pZWE ATF PAI2CD ,以其转化大肠杆菌JF112 5 ,经温度诱导 ,ATF PAI2CD获得较高水平表达 ,融合蛋白质以包涵体形式存在 ,占菌体总蛋白质的 15 %。
And take c|myc as example,it shows how to construct the eukaryotic expression vector which is used to express the fused protein,and how to detect and purify the fused protein by epitope tagging and its specific monoclonal antibody.
并以c myc标记肽为例 ,简述如何构建用于融合蛋白表达的植物表达载体 ,及如何利用标记肽及其特异性抗体表达并纯化融合蛋白质 。
