45 kb DNA fragment containing the 26S promoter of Semliki Forest virus(SFV) and a linker was amplified by PCR and inserted into the multiple clone sites of pSCA1,a suicidal DNA vaccine vector based on SFV replicon,resulting in a modified vector pSCA2 to drive the co-expression of double genes.
PCR扩增西门利克森林病毒(SFV)亚基因组启动子26S序列及人工合成Linker,将其插入基于SFV复制子的“自杀性”DNA疫苗表达载体pSCA1的多克隆位点,获得含2个26S启动子并能同时驱动双基因共表达的“自杀性”DNA疫苗表达载体pSCA2。
Construction and immunity response of a suicidal DNA vaccine with E gene of Japanese encephalitis virus in mice
乙型脑炎病毒E基因自杀性DNA疫苗的构建及其免疫应答
Suicidal DNA vaccine has become the focus of DNA vaccine researches not only advantages of conventional DNA vaccine,but also because of its advantages of biosafety and enhanced immunogenicity.
菌落PCR和酶切鉴定证明重组质粒pSMART-ORF5包含完整的ORF5基因,成功构建PRRSV自杀性DNA疫苗。
Research Progress and Application Status of Tumor Vaccines;
肿瘤疫苗研究进展及应用现状
Animal model of endometriosis treated with xenogeneic endothelial cells as vaccines in Lewis rats;
异种血管内皮细胞疫苗治疗Lewis大鼠子宫内膜异位症的动物模型
Analysis of Japanese B Encephalitis Vaccination and a Knowledge,Attitude,and Practice Survey in Shaanxi Province;
陕西省流行性乙型脑炎疫苗接种及知信行调查分析
Field trial of vaccination of channel catfish(Ictalurus punctatus) with Aquavac-ESC aganist enteric septicemia of catfish in China;
AQUAVAC-ESC疫苗免疫预防斑点叉尾鮰(Ictalurus punctatus)肠道败血症的田间试验
The Study of Vaccination of NogoA Combined with Olfactory Ensheathing Glia Implantation in Treatment of Acute Spinal Cord Injury;
NogoA疫苗联合嗅鞘细胞移植治疗急性脊髓损伤的实验研究
Researches on Suicidal DNA Vaccine of PRRSV and Its Deliverying with Bacterial Ghosts
猪繁殖与呼吸综合征病毒自杀性DNA疫苗及其菌蜕复合疫苗的研究
Suicidal DNA Vaccine of Avian Infectious Bronchitis Virus M Gene Induces Immune Response in Chicken;
禽传染性支气管炎病毒M基因自杀性DNA疫苗及免疫效应研究
Construction and immunity response of a suicidal DNA vaccine with E gene of Japanese encephalitis virus in mice
乙型脑炎病毒E基因自杀性DNA疫苗的构建及其免疫应答
Construction and Preliminary Immunogenicity Assess of Infectious Bronchitis Virus S1 Gene Suicidal DNA Vaccine Plasmid;
禽传染性支气管炎病毒S1基因自杀性DNA疫苗的研究
Study on Suicidal DNA Vaccine Encoding HA/NA of Avian Influenza Virus;
禽流感病毒HA/NA基因的“自杀性”DNA疫苗研究
Study on Inhibition of Japanese Encephalitis Virus Replication by Small Interfering RNAs and Suicidal DNA Vaccine of Japanese Encephalitis Virus;
应用siRNAs抑制乙型脑炎病毒复制及乙型脑炎“自杀性”DNA疫苗研究
The Immunogenicity of a Plasmid DNA Encoding Multiple-epitopes of Foot-and-Mouth Disease Virus;
口蹄疫多表位DNA疫苗的免疫原性
Construction and immunogenic evaluation of a DNA vaccine containing the human papillomavirus type 58 E2 gene
HPV58 E2 DNA疫苗的构建及免疫原性研究
The Immunogenicity and Safety Assessment of a Plasmid DNA Encoding Multiple-epitopes of Foot-and-Mouth Disease Virus;
口蹄疫多表位DNA疫苗的免疫原性和安全性研究
Studies on the SARS-CoV DNA Vaccines;
SARS-CoV DNA疫苗研究
Distribution and Safety Studies of IBV DNA Vaccine and DNA/Cationic Liposome Complex;
IBV DNA疫苗、DNA疫苗—脂质体复合物在鸡体内的分布及安全性研究
Safety Evaluation of DNA Vaccine Against Infectious Canine Hepatitis;
犬传染性肝炎DNA疫苗安全性研究
Experimental Study on HCV Therapeutic DNA Vaccine;
丙型肝炎治疗性DNA疫苗的实验研究
Construction and Immunogenicity Assessment of a Plasmid DNA Encoding Infectious Canine Hepatitis Virus;
犬传染性肝炎DNA疫苗的构建和免疫原性研究
A DNA Prime-rMVA Boost Vaccine Combinations for Simultaneous Induction of Humoral and Cell-Mediated Immunity;
HBV DNA疫苗和重组痘病毒联合疫苗的免疫原性研究
Study on Elicition of Anti-tumor Immunity by Particulate Peptide-DNA Dual Vaccine;
颗粒性肽-DNA复合疫苗激发抗肿瘤免疫的研究
Construction and Characterization of a Recombinant Live Vectored Vaccine and a DNA Vaccine Against Classical Swine Fever;
抗猪瘟重组活载体疫苗及DNA疫苗的构建及其生物学特性分析
Molecular design and immunogenicity of a multiple-epitope foot-and-mouth disease virus antigen,adjuvants,and DNA vaccination
口蹄疫病毒三价复合多表位佐剂DNA疫苗构建及其免疫原性