A 150-min short procedure for the determination of homocystine content was developed on the basis of the physioiogical properties of homocystine and the analytical method of physiological body fluid (Hitachi Model 835-50 Amino Acid Analyzer).
根据日立835—50型氨基酸自动分析仪生理体液分析法,以及同型胱氨酸的生理特性,建立测定同型胱氨酸的150min短程序法。
XRD results show that all ceramic samples are perovskite and the morphotropic phase boundary(MPB)is located in x=0.
5TiO3系压电陶瓷,XRD分析表明所得陶瓷样品为纯的钙钛矿结构,其准同型相界在x=0。
Objective To express and purify a single chain antibody (scFv)5C1 which specifically reacted to acute myelogenous leukemia cell line KG1a cell.
方法 基因重组构建表达 5C1scFv的载体pSTE 5C1,在大肠杆菌中诱导表达 ,金属离子螯和亲和层析法纯化 ,获得高纯度的活性 5C1scFv ;借助生物素 链亲和素的高亲和力和高灵敏度的显色系统 ,用Westernblot分析其识别的KG1a细胞膜蛋白的Mr;用聚集实验分析 5C1scFv对KG1a细胞同型聚集的影响。
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