Qiufeng M, a new green-revertible albino mutant, was used in this study.
水稻白化转绿突变体是一类新的叶色突变体材料。
Methods A synonymous mutation and a hybrophobic polypeptide linker(Gly_(4)Ser)_(3) were used to splice three different gene fragments of β2m and HLA-A2 extracellular region subunits by overlap-PCR for constructing single chain β2m-HLA-A2-BSP fusion gene in vitro.
方法应用重叠延伸PCR(overlap-PCR)对编码HLA-A2的cDNA序列进行一个碱基的同义突变,并将β2m和HLA-A2胞外段亚单位两段编码序列的cDNA通过一疏水性多肽接头(Gly4Ser)3的DNA序列进行前后拼接,并利用引物所带的BSP编码序列,构建单链β2m-HLA-A2-BSP融合基因,将测序正确的β2m-HLA-A2-BSP融合基因插入原核表达载体pET-22b中,转化BL21(DE3)细菌,SDS-PAGE检测其表达,融合蛋白于体外抗原肽存在条件下稀释复性后由Western blot鉴定其空间构象。
The Physiological Character and Gene Mapping in a New Green-Revertible Albino Rice Mutant;
一个新的水稻白化转绿突变体的生理特性及基因定位
CHARACTERISTIC,INHERITANCE AND BREEDING APPLICATION OF RICE MUTANTS WITH GREENABLE ALBINO LEAF
水稻白转绿突变体的特性、遗传及其育种应用
Study on the Developmental Characters of Chloroplasts in a Yellow Type Mutant of Oilseed Rape Seedlings;
油菜黄化突变体叶绿体发育特性研究
QTL Analysis for Traits Associated with Photosynthetic Functions and Map-based Cloning of Virescent White Leaf Gene in Rice (Oryza Sativa L.);
水稻光合功能相关性状QTL分析及转绿型白叶突变体基因的图位克隆
Photosynthetic Characteristics and Chloroplast Proteins Analysis of Leaf Color Mutants of Biao 810S;
水稻叶色突变体标810S光合特性及叶绿体蛋白质分析
Cloning and Transformation of lba Gene of Glycine max into Chloroplast of Chlamydomonas reinhardtii
豆血红蛋白基因lba的克隆及其转化衣藻叶绿体
Construction of the mutant of human amyloid precursor protein and enhanced fluorescence protein fusion gene
突变型人APP基因与增强型绿色荧光蛋白基因融合载体的构建
The Anatomical Character of Split Rice Glume Mutant and Preliminary Orientation of Leaf s Yellowish-green Gene;
水稻裂颖突变体的解剖特征和叶片黄转绿基因的初步定位
Construction of Plant Expression Vector with Mutation Gene of Pokeweed Antiviral Protein and the Study of Its Genetic Transformation in Rice;
美洲商陆抗病毒蛋白突变体表达载体的构建及农杆菌转化水稻的初步研究
Optimization of Agrobacterium-Mediated Transformation of Cotton and the Development of Mutants;
棉花遗传转化体系的优化及突变体的创制
Purification of Human Mutant CD59 Protein and Preparation of Monoclonal Antibody;
人突变CD59蛋白的提取纯化与单克隆抗体的制备
Genetic Analysis and Gene Mapping on a Rice Albino Mutant XGS;
一份水稻白化突变体的遗传分析及其基因定位
Isolation and Map-based Cloning of Albino Mutant Ems15 in Arabidopsis Thaliana
拟南芥白化突变体ems15的筛选和基因定位
Prokaryotic expression, purification and identification of human mutant nm23-H1 protein
人重组蛋白nm23-H1突变体原核表达纯化与鉴定
Construction of N-linked glycosylation site mutants of envelope glycoprotein genes of hantavirus
汉坦病毒包膜糖蛋白糖基化位点突变体的构建
Screening of the Mutations Induced by the Transposon Activation Construct in Land Cotton;
转座活化结构诱发的棉花突变体的筛选
Agrobacterium tumefaciens-mediated Transformation of Alternaria alternata and Identification of Hypovirulent Mutant
链格孢ATMT转化体系的构建及弱毒突变株验证
Research and Aplication of the Greenish-Yellow Bud Mutant in Hot Pepper;
辣椒黄绿苗突变体特异种质应用研究
