Objective To establish the screening method of detecting genetically modified food in food stuffs by PCR amplifying the camv 35?S pr omoter and nos terminator.
方法 用改良溴化十六烷三甲基铵 (CTAB)法制备转基因抗除草剂〔大豆RoundUpReadyTMSoybean(RRS)〕和转基因抗虫玉米系列标准品Bt176Maximaizer的DNA ,PCR检测其内参照基因及camv 35S启动子和nos终止子。
Methods The presence of GMO were investigated by PCR detection of camv 35S promoter and nos terminator, and the presence of RoundUp Ready TM Soybean (RRS), Bt176 Maximaizer or Mon810 YieldGard in GMO-positive samples were further determined by PCR detecting their specific DNA fragments respectively.
方法通过PCR检测camv35S启动子和nos终止子,筛选食品中是否含转基因成分,并进一步通过PCR检测RoundUpReadyTMSoybean(RRS)、Bt176Maximaizer和Mon810YieldGard的特异DNA片段,判断是否含这三种成分。
The fragment of NOS terminator was obtained through the operating of DNA isolation and polymerase chain reaction(PCR)amplification from genetically modified tobacco.
以转基因烟草为材料,通过DNA提取、PCR扩增获得NOS终止子片段产物,以重叠延伸扩增法制备该片段的竞争性模板,并进行酶切验证。
The growth promoting activity of two different kinds of reconstructed terminators in transgenic common carp
两种不同终止子在转基因鲤鱼中的促生长效应
Using three pairs of specific primers, DNA fragments containing the promoter of 16S rRNA gene and the promoter and terminator of psbA gene, were obtained by PCR from Populus tomentosa chloroplast genomic DNA.
从毛白杨(Populus tomentosa)组培苗中提取基因组总DNA,用PCR方法克隆了包含psbA基因启动子、终止子和16SrRNA基因启动子的叶绿体DNA序列,并在此基础上克隆了启动子Prrn、PpsbA及终止子TpsbA。
Function of the T_(32) Terminator in Gene Expression in Corynebacteria;
T_(32)终止子在棒状杆菌基因表达中的功能
The growth promoting activity of two different kinds of reconstructed terminators in transgenic common carp
两种不同终止子在转基因鲤鱼中的促生长效应
Does the proton finally come to rest?
这个质子最终会静止吗？
Termination of free radical chairs occurs by a bimolecular reaction.
自由基链的终止产生于双分子反应。
Now there is a new government tool to help stop unwanted calls.
目前政府有新法子协助终止推销电话。
After arteria uterina involvment treatment termicnation tubal pregnancy
经子宫动脉介入治疗终止输卵管妊娠
Clinical observation of SAU new method in the termination of unwanted pregnancy with scars womb
SAU新方法终止瘢痕子宫意外妊娠临床观察
Time and method of pregnancy termination in severe preeclampsia and the maternal-perinatal outcome
重度子痫前期终止妊娠时机及妊娠结局分析
chain termination method
链终止（DNA测序）法
terminate a pregnancy,eg by means of an abortion
终止妊娠（堕胎）.
A progression of chords moving to a harmonic close or point of rest.
终曲，终止式和弦进行到和谐的终曲或终止乐句
Initializer list must be terminated by
预设值列表必须用终止符终止
An expiration, especially of a contract or an agreement.
满期终止,特别是合同或协议的终止
Involuntary terminations must be reported concurrent with the termination.
偶然终止必须告知发生终止的事件。
If enough molecules cease to vibrate in a given part of the form, the cells die.
如果在形态的某部分中足够多分子终止振动，那么细胞死亡。
To call a subroutine to terminate the reading from or writing to a file by a program.
调用一个子程序来终止程序对一个文件的读出或写入。
Their wives, too, were unhappy for the same reasons and gave up their social activities.
他们的妻子也同样为此郁郁不乐，终止了一切社会活动。
Children's duty to maintain their parents shall not terminate with the change in their parents" matrimonial relationship.
子女对父母的赡养义务，不因父母的婚姻关系变化而终止。
