Objective To establish the screening method of detecting genetically modified food in food stuffs by PCR amplifying the camv 35?S pr omoter and nos terminator.
方法 用改良溴化十六烷三甲基铵 (CTAB)法制备转基因抗除草剂〔大豆RoundUpReadyTMSoybean(RRS)〕和转基因抗虫玉米系列标准品Bt176Maximaizer的DNA ,PCR检测其内参照基因及camv 35S启动子和nos终止子。
Methods The presence of GMO were investigated by PCR detection of camv 35S promoter and nos terminator, and the presence of RoundUp Ready TM Soybean (RRS), Bt176 Maximaizer or Mon810 YieldGard in GMO-positive samples were further determined by PCR detecting their specific DNA fragments respectively.
方法通过PCR检测camv35S启动子和nos终止子,筛选食品中是否含转基因成分,并进一步通过PCR检测RoundUpReadyTMSoybean(RRS)、Bt176Maximaizer和Mon810YieldGard的特异DNA片段,判断是否含这三种成分。
The fragment of NOS terminator was obtained through the operating of DNA isolation and polymerase chain reaction(PCR)amplification from genetically modified tobacco.
以转基因烟草为材料,通过DNA提取、PCR扩增获得NOS终止子片段产物,以重叠延伸扩增法制备该片段的竞争性模板,并进行酶切验证。
