Methods The cell culture technique,the Hemagglutination reaction and MTT test were used to determine the effects of MXSGD on hemagglutination titer of influenza virus A and MDCK cells activities infected by influenza virus A with 4 different treatment methods.
方法运用细胞培养技术、血凝试验和MTT法检测麻杏石甘汤在4种给药方式中对流感病毒血凝滴度和流感病毒感染的MDCK细胞活性的影响。
Methods Influenza A (H3N2, H1N1) and B virus were inoculated in MDCK cells, MEK cells and FRhK-4 cells under different culture conditions to observe cytopathic effect (CPE), hemagglutination titer.
方法采用MEK细胞、FRhK-4细胞、MDCK细胞分别接种甲型流感病毒H1N1型、H3N2型和乙型流感病毒疫苗毒株,观察3种毒株在不同培养条件下的细胞病变、血凝滴度。
Methods Madin darby canin kidney (MDCK) cell culture techniques were used for evaluating antiviral activity of ACT by hemagglutination assay with the influenza virus hemagglutination titer as index and for investigating the inhibitory effect of ACT on replication of influenza virus.
方法 采用 MDCK细胞培养法 ,通过红细胞凝集实验 ,以流感病毒血凝滴度为指标 ,观察 ACT对流感病毒复制的抑制作用。
