Screening the inhibitors for PrA from Ganoderma lucidum by immobilized enzyme affinity chromatography;
固定化亲和层析分离灵芝发酵液蛋白酶A抑制剂
Purification of Glutathione (GSH) by metal-chelated affinity chromatography;
金属螯合亲和层析分离纯化谷胱甘肽(GSH)初探
A new technology of isolation and purification:affinity chromatography;
分离纯化新技术亲和层析
Purification of NGF from Naja naja atra Cantor venom by immunoaffinity chromatography WEI Chuan-bao, HUANG Ya-nan;
舟山眼镜蛇毒神经生长因子的亲和层析分离
Immunoaffinity chromatography was used to purify rhI.
用BmNPV/IGF 1感染家蚕幼虫 ,提取家蚕血淋巴液 ,采用亲和层析法纯化rhIGF 1,ELISA法测定rhIGF 1含量 ,用SDS PAGE和Western blot分析鉴定rhIGF 1纯度及免疫学活性 ,MTT法观察其对MCF 7细胞增殖的影响。
coli BL21and myostatin fusion protein was purified by affinity chromatography.
为研究猪肌生成抑制素(myostatin,MSTN)蛋白的功能及为制备此蛋白的单克隆抗体提供材料,将原核表达菌种MSTN-PGEX-4T-1-BL21进行融合表达,用亲和层析的方法对GST-MSTN和MSTN C-端成熟蛋白进行分离纯化。
The development of affinity chromatography technique is very fast in recent years,remarkable ac-complishments have been achieved in the separation and purification of biomolecules and organisms as well as in studying the interactions between biomolecules.
近几十年来,亲和层析技术发展十分迅速,广泛应用于生物分子(如结合蛋白、酶、抑制剂、抗原、抗体、激素、激素受体、糖蛋白、核酸及多糖类等)及组织(如细胞、细胞器、病毒等)的分离和纯化,是蛋白质组学研究中重要的技术之一。
Based on their specificity, Sepharose CL-6B was adopted in this study as affinity chromatography to separate the S-type lectin in annelid from the earthworm, in which EDTA-MEPBS (2 mmol/L EDTA, 4 mmol/Lβ—mercaptoethanol, 150 mmol/L NaC1, 20 mmol/L phosphate, pH7.
根据其性质，本研究采用惰性分子筛填料Sepharose CL-6B作为亲和层析介质对蚯蚓S型凝集素进行了纯化。
Bind Resin Chromatography under denaturing condition and dialyzed for renaturation, and then were analyzed with SDS-PAGE, West.
目的探讨通过金属螯合亲和层析的方法获得高纯度的人内皮细胞高表达脂多糖相关因子1(EOLA1)蛋白的可行性。
The fusion protein was purified by chromatography Glutathione Sepharose 4B and digested by 3C protease.
用glutathioneSepharose 4B亲和层析柱纯化表达产物后 ,以重组 3C蛋白酶对融合蛋白进行解离 ,SDS PAGE对表达及纯化产物进行鉴定。
panning technique
淘选技术[如通过亲和层析纯化细胞]
Purification of Rabbit Hepatic Glutathione Peroxidase by Affinity chromatography
亲和层析纯化兔肝谷胱甘肽过氧化物酶
Application and Development of Affinity Chromatography in Biological Science
亲和层析技术在生物科学中的应用及发展
Purification by Affinity Chromatography and Characterization of Tea Leaf β-Glucosidase
茶叶β-葡萄糖苷酶亲和层析纯化与性质研究
Isolation of Carbohydrate-deficient Transferrin (CDT) by Allo-A Sepharose 4B Affinity Chromatography;
Allo-A Sepharose 4B亲和层析分离血清糖缺失转铁蛋白
Purification of hCG Monoclonal Antibodies using rProtein A affinity Chromatography
应用蛋白A亲和层析法纯化hCG单克隆抗体
Preparation of high purity aprotinin by globate cross-linked chitosan
壳聚糖微球亲和层析提取抑酶肽的研究
Purification of recombinant lysostaphin by monoclonal antibody affinity chromatography
单克隆抗体亲和层析法纯化重组溶葡萄球菌酶
Prepare and Characterize Galactose-binding Proteins in Plant Seeds Using Sepharose CL-6B Affinity Chromatography;
利用琼脂糖凝胶CL-6B亲和层析纯化、分析植物半乳糖结合蛋白
Purification of Porcine Pancreas Kallikrein by Ion-Exchange and Affinity Chromatography;
离子交换和亲和层析技术纯化制备猪胰激肽释放酶
APPLICATION OF LECTIN AFFINITY CHROMATOGRAPHY OF SERUM ALKALINE PHOSPHATASE IN THE DIFFERENTIAL DIAGNOSIS OF LIVER DISEASES
血清碱性磷酸酶的凝集素亲和层析在肝脏疾病鉴别诊断中的应用
Studies on the Sugar Chain Structure of Alkaline Phosphatase in Human Sera Using Lectin Affinity Chromatography
凝集素亲和层析研究人血清肝型碱性磷酸酶的糖链结构
Organic-inorganic Hybrid Hollow Spheres Applied in Biologic Affinity Separation;
多孔二氧化硅空心微球的制备及其在生物亲和层析中的应用
Purification of Nerve Growth Factor from Guangxi Cobra Venom by Affinity Chromatography and Its Study on Antitumor Function;
广西眼镜蛇毒神经生长因子的亲和层析分离及其抗肿瘤作用
Purification by MCAC and Molecular Immobilization of SOD from Maize;
金属螯合亲和层析纯化玉米SOD及其修饰与固定化
Preparation Monoclonal Antibody of Anti-Shiga Toxin ⅡB and Using for Immunoaffinity Chromatography;
抗志贺毒素ⅡB亚基（Stx2B）单抗的研制及其亲和层析的应用
Study on Purification of Membrane Protein by Immuno-affinity Chromatography and Indirect ELISA for Detection of Mycoplasma Hyopneumoniae;
猪肺炎支原体膜蛋白免疫亲和层析及ELISA检测方法的建立
Preparation of Monoclonal Antibody Against Antigen Associated Human Lung Cancer and the Immunoaffinity Chromatography Purification of Its Antigen;
人肺癌相关抗原单克隆抗体的制备及其识别抗原的亲和层析纯化
