Cloning and phylogenetic analysis of bovine prochymosin gene;
牛凝乳酶原基因克隆及序列的进化分析
The Kpn I-Kpn I fragment of prochymosin gene was inserted into the phagemidvector:pBluescript.
以ｐｈａｇｅｍｉｄ，ｐＢｌｕｅｓｃｒｉｐｔ为载体，把牛凝乳酶原基因的ＫｐｎＩ－ＫｐｎＩ片段插入，将牛凝乳酶４５位Ｃｙｓ用Ａｌａ取代，根据遗传密码子的兼并性，在其附近引入限制性内切酶ＣｌａＩ位点。
During the work of site\|directed mutagenesis at disulfide bond Cys206\|Cys210 of prochymosin,it was found that the corresponding template sequence had the potential to form a loop\|stem structure with free energy of -16 1 kcal/mol,which prevent the template from pairing with primer and,in turn,the synthesis of the mutated DNA strand.
在对凝乳酶原二硫键Cys2 0 6 Cys2 10进行定位突变过程中发现 ,在相应的模板序列中有自身形成自由能为 - 16 1kcal/mol的茎环结构倾向 ,妨碍与引物结合 ,从而难以合成突变的DNA ,采用快退火可解决此矛盾。
By comparing PDI assisted renaturation with GSH/GSSG assisted renaturation of recombinant prochymosin, we found that: (1)Although the reaction rate of GSH/GSSG assisted renaturation was lower than that of PDI assisted renaturation, there was no difference between the final renaturation efficiencies of them.
对蛋白质二硫键异构酶与ＧＳＨ／ＧＳＳＧ促进重组凝乳酶原复性进行了比较。
Then based on blast the sequence of Ovis aries preprochymosin cDNA (Accession No: X53037) reported in GeneBank, two pairs of primers were designed to clone Xinong Saanen Goat preprochymosin with the 5 d postparturition abomasum cDNA as template by RT-PCR method.
本研究参照GenBank收录的绵羊凝乳酶原前体cDNA序列(GenBank No:X53037)设计RT-PCR、PCR与巢式引物,以新生5天的西农萨能羊公羔羊为研究对象,利用手术法采集羔羊皱胃组织,利用Trizol法提取皱胃组织总RNA,经过电泳鉴定后,以总RNA为模板利用RT-PCR方法获得西农萨能羊凝乳酶原前体cDNA序列,以cDNA序列为模板进行扩增和T载体连接测序。
Cloning and Bioinformatic Analyses of Preprochymosin of Xinong Saanen Goat and Construction of Eukaryotic Expression;
西农萨能羊凝乳酶原前体基因的克隆、生物信息学分析及真核表达载体构建
rennet-digested milk
用凝乳酶凝结的乳凝块
An enzyme, such as rennin or thrombin, that induces coagulation.
凝固酶能引起凝固作用的一种酶，如凝乳酶和凝血酶
tissue thromboplasti
组织促凝血酶原激酶
thromboplastin antecedent
促凝血酶原激酶先质
phosphoglucomutase and chymotrypsi
磷酸葡萄糖变位酶与胰凝乳蛋白酶
sweetened milk coagulated with rennet.
加糖的牛奶，使用凝乳酶使之凝结。
congenital aprothrombinemia
先天性凝血酶原缺乏症
Effect of rennet on producing Quark cheese by ultrafiltration concentrated milk
凝乳酶对超滤浓缩乳生产Quark干酪的影响
an enzyme that occurs in gastric juice; causes milk to coagulate.
胃液中的一种酶，能使乳类凝结。
chymopapain (INN)
木瓜凝乳蛋白酶（国际非专利药品名称）
Food ingredients such as recombinant rennet (ehymosin) have also been launched in the marketplace.
食品成分,如重组凝乳酶已投入市场。
Study on Immobilized α-chymotrypsin Based on PNIPAM/HEMA;
PNIPAM/HEMA固定化α-胰凝乳蛋白酶的研究
Studies on Factors Affecting of the Production of a Milk-Clotting Enzyme from Mucor Pusillus and Its Recombinant Enzyme, and Their Enzymatic Properties
微小毛霉凝乳酶及其重组酶的产酶条件和酶学特性的研究
Chymotrypsin attacks the same peptide bonds that pepsin does.
胰凝乳蛋白酶和胃蛋白酶分解同一类肽
Studies on Screening of Bacterial Strains Producing Chymosin and Its Enzyme Properties;
凝乳酶优良菌株的选诱及其酶活特性的研究
Study on Factors of Producing the Milk Clotting Enzyme from Mucor Pusillus and Enzyme Characterization;
微小毛霉产凝乳酶条件及酶性质的研究
Study on Partial Characterization of Kid Rennet and Microbease
羔羊皱胃酶和微生物凝乳酶部分特性的探讨
