To investigate the differences of recombinant vectors introducing ubiquitous expression and tissue-specific expression in Caenorhabditis elegans,the core promoter region of Act-1 gene and T07F10 gene,and the Poly(A) signal region of T07F10 gene were amplified from the genomic DNA of C.
以克隆载体pBluescript SK+为基本骨架,分别构建由Act-1基因的核心启动子序列、T07F10基因的核心启动子序列调控的、EGFP作为报告基因的秀丽隐杆线虫全身性表达重组载体和组织特异性表达重组载体,为研究不同类型载体在秀丽隐杆线虫体内的表达模式提供基础。
