sinensis s optic ganglia was compared by 2-DE.
利用双向电泳技术比较正常和性早熟河蟹视神经节蛋白的表达差异,旨在探索蛋白质组学技术在甲壳动物中尤其是河蟹的性早熟研究当中的应用。
Five analytical approaches,such as the precipitation separation of TCA/ acetone,the buffer extraction,the column chromatography,the lyolysis buffer,and the lyolysis-ultracentrifugation,were compared to extract and separate the protein from the optic ganglion of Sepia esculenta,respectively.
选用TCA/丙酮沉淀法、缓冲液法、柱层析法、裂解液法和裂解液-超速离心法分别提取金乌贼视神经节蛋白质,其中蛋白质得率较高的方法有裂解液-超速离心法、裂解液法,其次为TCA/丙酮沉淀法。
The results showed that:ER-immunoreactive substance was located in medulla externa,medulla interna and medulla terminalis in optic ganglion.
利用免疫细胞化学SABC法对锯缘青蟹(Scylla serrata)视神经节雌激素受体(ER)进行定位研究。