The hLF gene was inserted into the baculovirus transfer vector pBacPAK_8, and the recombinant plasmid pBacPAK-hLF was co-transfected with linearized Bm-BacPAK_6 virus DNA into BmN cells, then homologous recombination occurred inside the cells.
该质粒DNA与已线性化BacPAK6DNA共转染家蚕细胞BmN,在培养的贴壁细胞中挑出空斑,经过3轮纯化,获得重组病毒BmNPV-hLF。
In order to deal with this problem,we cloned the poly gene with its promoter of Bombyx mori nucleopolyhedrovirus(BmNPV) into pigA3GFP vector,and transported it into BmN cells by lipofectamine with helper vector pHA3PIG.
将家蚕核型多角体病毒(Bombyx mor inucleopolyhedrovirus,BmNPV)中的多角体基因(polyhedrin,poly)及其启动子片段克隆到转座子载体pigA3GFP中,将其与辅助质粒pHA3PIG利用脂质体介导法导入家蚕细胞中,经过多次筛选获得稳定的转基因家蚕细胞。
Constructed vector was coinfected with linear Bm BacPAK6 DNA into BmN cells.
与线形化Bm BacPAK6病毒基因组DNA共转染家蚕细胞,经过体内重组,筛选到重组病毒Bm BacPAK IFN THY。
The recombinant BmNPV(rBmHBe) was used to study the optimal conditions for expression of HBeAg gene in BmN cell.
利用已构建的含有HBeAg基因的重组家蚕核多角体病毒rBmHBe ,摸索了HBeAg在家蚕细胞中表达的最佳条件组合 ,细胞接种密度 :(3 。
It was widely used by cytologists to stain chromosomes.
它被细胞学家广泛地用来着色染色体。
Studies on the Cellular Pathology of DNV-like and the Establishment of an Embryonic Cell Line from Silkworm;
家蚕类浓核病毒的细胞病理学研究及家蚕胚胎细胞系SWAU1-BmE的建立
cell theory and classification
细胞学说及细胞分类
Adult stem cells are thought by scientists to be less versatile than their embryonic equivalents.
在科学家看来，成人干细胞没有胚胎干细胞那样神通广大。
Cellular Basis of Abnormal Calcium Cycling of Failing Rabbits Myocytes and Effects of Metoprolol and Losartan;
家兔心衰心肌细胞钙循环异常的细胞学基础及药物干预研究
For the first time ever, scientists have been able to create a chemical element that can fight cancer cells.
科学家们首次研制出了抵抗癌细胞的化学元素。
The reason embryonic stem cells are valued by scientists is that the cells' descendants can turn into any other sort of body cell.
科学家们认为胚胎干细胞有价值是因为，胚胎干细胞可以发育成为任何其他种类的体细胞。
Scientists can easily genetically alter adult cells.
科学家能很容易地利用遗传方法改变成体细胞。
Scientists used to think that the number of cells in a human brain is fixed at birth.
过去科学家认为，人的脑细胞数终生不变。
However, few people keep cell-culture medium handy at home or in school.
然而，在家里或学校里很少人手边有细胞培养液。
Study on die Genetics of Basal Cell Nevus Syndrome in One Family
一家族基底细胞痣综合征遗传学的研究
DISTRIBUTION AND MORPHOLOGIC OBSERVATION OF ARGYROPHIL CELLS IN INTESTINES OF COLUMBA LIVIA DOMESTICA
家鸽肠道嗜银细胞的分布及形态学观察
Establishment of cell line of Musca domestica embryo
家蝇胚胎细胞系的建立及其生物学特性
Cytology The study of cells; cell biology.
细胞学：研究细胞的学科，又称细胞生物学。
(biology) nuclear membrane.
（生物学）（细胞）核膜。
The Morphologic and Immunohistochemical Studies on Alimentary Canal of Wolves and Domestic Dogs;
狼与家犬消化道形态学及其消化道内分泌细胞免疫组织化学研究
Scientist will use the surplus stem cells already created for treatment of infecundity for their research.
在胚胎干细胞研究中，科学家从不孕症治疗所留下的胚胎取得干细胞。
Scientists can then genetically alter these cells and find which ones did transform and then clone only those cells.
然后，科学家能遗传性地改变这些细胞并找出确能转型的细胞进行克
